Human × Mouse Somatic Cell Hybrid Clone secreting Immunoglobulins of both Parental Types

  title={Human × Mouse Somatic Cell Hybrid Clone secreting Immunoglobulins of both Parental Types},
  author={Jerrold Schwaber and Edward P. Cohen},
HYBRIDS between two distinct differentiated cell types usually do not express the differentiated characteristics of either parental cell. The synthesis of enzymes necessary for the metabolism of the cell continues, while the synthesis of specialized products of differentiation often is suppressed1. Mouse myeloma cells, after fusion with mouse fibroblasts, follow this pattern ; that is, the hybrid cells cease synthesis of immunoglobulin2,3. A myeloma × lymphoma hybrid, however, continues… 
Immunoglobulin production by a human-mouse somatic cell hybrid.
  • J. Schwaber
  • Biology, Medicine
    Experimental cell research
  • 1975
The fusion of human lymphocytes and TEPC-15 mouse myeloma cells, which had not been adapted to culture, resulted in the establishment of in vitro hybrid cell cultures, which resulted in ten clones of this somatic cell hybrid that had the mouse parental histocompatibility antigens but only four clones also retained the human parental histOCompatibilityAntigens.
Human-human hybrids secreting pneumococcal antibodies.
A human myeloma "analog" (LSM 2.7) is constructed which supports the synthesis and secretion of human immunoglobulin in vitro upon fusion with human peripheral blood and spleen mononuclear cells.
Induction of human immunoglobulin synthesis and secretion in somatic cell hybrids of mouse myeloma and human B lymphocytes from patients with agammaglobulinemia
The studies demonstrate the presence of functional structural genes coding for human immunoglobulin heavy chains in B lymphocyte of patients with agammaglobulinemia and represent induction in the somatic cell hybrids of a gene product not expressed in the parental B lymphocytes.
Phenotype of hybrids between lymphoid cells and rat hepatoma cells
The lymphoid phenotype, as monitored by production of kappa light chains specified by the diploid and tetraploid lymphoid partner cells, was totally suppressed within 72 h after fusion, and no difference in phenotypic expression was observed, whether the hybrid cells were grown as monolayer or as suspension cultures.
The construction and use of a human-mouse myeloma analogue suitable for the routine production of hybridomas secreting human monoclonal antibodies.
A human-mouse myeloma analogue termed HMMA2.11TG/O was constructed by fusion of the mouseMyeloma cell line P3x63Ag8.653 with bone marrow mononuclear cells from a patients with IgAmyeloma and should prove useful for the routine production of human monoclonal antibodies.
Characterization of two mouse myeloma X sheep lymphocyte cell lines secreting sheep antibody.
Two mouse X sheep interspecific cell hybrids were obtained by fusing mouse myeloma cell line Sp2/O with sheep lymphocytes obtained from a lymph node antigenically stimulated with azo-benzene arsonate-ovalbumin (ABA-ova) using immunochemical, karyotypic and molecular DNA techniques.
Human lymphocyte hybridomas and monoclonal antibodies.
This chapter reviews the accumulating data concerning human monoclonal antibodies and hybridomas and outlines possible future directions of human monOClonal antibody research and development.
The efficient production of stable, human monoclonal antibody-secreting hybridomas from EBV-transformed lymphocytes using the mouse myeloma X63-Ag8.653 as a fusion partner.
It is concluded that the mouse myeloma X63-Ag8.653 is a suitable fusion partner with EBV-transformed B cells in the efficient production of human monoclonal antibodies.
Regulation of immunoglobulin expression in mouse myeloma cells.
The genetic information obtained by isolating somatic cell hybrids of mouse myeloma cells expressing different classes and subclasses of immunoglobulin is reviewed.
Strategies for Stable Human Monoclonal Antibody Production
The development of hybridoma technology by Kohler and Milstein (1975) opened a new era not only in immunology, but in all fields of biological science, where the availability of human monoclonal antibodies would be advantageous.


IgG Synthesis in Hybrid Cells from an Antibody-producing Mouse Myeloma and an L Cell Substrain
An investigation of the synthesis of immunoglobulin by clones of these hybrid cell strains produced using Sendai virus to mediate fusion of a cloned, culture-adapted mouse plasmacytoma with the thymidine kinase deficient cell strain LM(TK−) C1 1D is reported.
Immunoglobulin G and free kapa-chain synthesis in different clones of a hybrid cell line.
  • B. Mohit
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1971
13 clonal cell lines were isolated from a hybrid cell population established by cell fusion between cloned BALB/c myeloma cells that were resistant to 8-azaguanine and produced immunoglobulin G and
Hybrid Cell Line from a Cloned Immunoglobulin-Producing Mouse Myeloma and a Nonproducing Mouse Lymphoma
A hybrid cell line was established by cell fusion between a cloned Balbic myeloma that is resistant to 8-azaguanine and produces immunoglobulin (γG and free kappa chain) and C57BL/6N lymphoma that is
Suppression of immunoglobulin synthesis by cellular hybridization.
Another example of redifferentiation in hybrid cells is reported; when IT mouse fibroblasts are fused with MPC 11 mouse myeloma cells which produce immunoglobulin, the hybrids neither produce nor excrete detectable amounts of immunoglOBulin.
The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time through the expedient of varying multiplicities of the parental cells and the total cell density.
Human-Mouse Somatic Cell Hybrids with Single Human Chromosome (Group E): Link with Thymidine Kinase Actvity
The hybrid cells have only mouse electro-phoretic variants for glucose-6-phosphate dehydrogenase, lactate dehyd nitrogenase, and malate dehydrogensase, suggesting that the human genetic loci for these enzymes are not represented in the hybrid genome and may be unlinked to that for thymidine kinase.
The formation of immunoglobulins by human tissues in vitro. I. The methods and their specificity.
From control experiments, it can be concluded that the labelling of the immunoglobulins is based on the incorporation of [14C]amino acids during incubation in vitro.
Chromosome preparations of leukocytes cultured from human peripheral blood.
Abstract A combination of cytological and leukocyte culture techniques is described which constitutes a convenient, reliable approach for chromosome studies of humans and yields the following
Induction in vitro of Hapten Specific Plaque Forming Cells
THE method developed by Mishell and Dutton1 for the in vitro stimulation of an apparently primary response to red cell antigens offers advantages over in vivo experiments in analysing the cellular
Hybridization of Somatic Cells
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