Human × Mouse Somatic Cell Hybrid Clone secreting Immunoglobulins of both Parental Types

@article{Schwaber1973HumanM,
  title={Human × Mouse Somatic Cell Hybrid Clone secreting Immunoglobulins of both Parental Types},
  author={Jerrold Schwaber and Edward P. Cohen},
  journal={Nature},
  year={1973},
  volume={244},
  pages={444-447}
}
HYBRIDS between two distinct differentiated cell types usually do not express the differentiated characteristics of either parental cell. The synthesis of enzymes necessary for the metabolism of the cell continues, while the synthesis of specialized products of differentiation often is suppressed1. Mouse myeloma cells, after fusion with mouse fibroblasts, follow this pattern ; that is, the hybrid cells cease synthesis of immunoglobulin2,3. A myeloma × lymphoma hybrid, however, continues… 

Induction of human immunoglobulin synthesis and secretion in somatic cell hybrids of mouse myeloma and human B lymphocytes from patients with agammaglobulinemia

The studies demonstrate the presence of functional structural genes coding for human immunoglobulin heavy chains in B lymphocyte of patients with agammaglobulinemia and represent induction in the somatic cell hybrids of a gene product not expressed in the parental B lymphocytes.

The construction and use of a human-mouse myeloma analogue suitable for the routine production of hybridomas secreting human monoclonal antibodies.

A human-mouse myeloma analogue termed HMMA2.11TG/O was constructed by fusion of the mouseMyeloma cell line P3x63Ag8.653 with bone marrow mononuclear cells from a patients with IgAmyeloma and should prove useful for the routine production of human monoclonal antibodies.

Strategies for Stable Human Monoclonal Antibody Production

The development of hybridoma technology by Kohler and Milstein (1975) opened a new era not only in immunology, but in all fields of biological science, where the availability of human monoclonal antibodies would be advantageous.

Human monoclonal antibodies

The EBV-hybridoma system retains the advantageous features of the other two systems while overcoming their pitfalls and may be the current method of choice for producing human monoclonal antibodies with a defined specificity.
...

References

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IgG Synthesis in Hybrid Cells from an Antibody-producing Mouse Myeloma and an L Cell Substrain

An investigation of the synthesis of immunoglobulin by clones of these hybrid cell strains produced using Sendai virus to mediate fusion of a cloned, culture-adapted mouse plasmacytoma with the thymidine kinase deficient cell strain LM(TK−) C1 1D is reported.

Immunoglobulin G and free kapa-chain synthesis in different clones of a hybrid cell line.

  • B. Mohit
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1971
13 clonal cell lines were isolated from a hybrid cell population established by cell fusion between cloned BALB/c myeloma cells that were resistant to 8-azaguanine and produced immunoglobulin G and

Hybrid Cell Line from a Cloned Immunoglobulin-Producing Mouse Myeloma and a Nonproducing Mouse Lymphoma

A hybrid cell line was established by cell fusion between a cloned Balbic myeloma that is resistant to 8-azaguanine and produces immunoglobulin (γG and free kappa chain) and C57BL/6N lymphoma that is

Suppression of immunoglobulin synthesis by cellular hybridization.

Another example of redifferentiation in hybrid cells is reported; when IT mouse fibroblasts are fused with MPC 11 mouse myeloma cells which produce immunoglobulin, the hybrids neither produce nor excrete detectable amounts of immunoglOBulin.

CONTROLLED PRODUCTION OF PROLIFERATING SOMATIC CELL HYBRIDS

The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time through the expedient of varying multiplicities of the parental cells and the total cell density.

Human-Mouse Somatic Cell Hybrids with Single Human Chromosome (Group E): Link with Thymidine Kinase Actvity

The hybrid cells have only mouse electro-phoretic variants for glucose-6-phosphate dehydrogenase, lactate dehyd nitrogenase, and malate dehydrogensase, suggesting that the human genetic loci for these enzymes are not represented in the hybrid genome and may be unlinked to that for thymidine kinase.

The formation of immunoglobulins by human tissues in vitro. I. The methods and their specificity.

From control experiments, it can be concluded that the labelling of the immunoglobulins is based on the incorporation of [14C]amino acids during incubation in vitro.

Induction in vitro of Hapten Specific Plaque Forming Cells

THE method developed by Mishell and Dutton1 for the in vitro stimulation of an apparently primary response to red cell antigens offers advantages over in vivo experiments in analysing the cellular

Hybridization of Somatic Cells

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