How valid are denaturant-induced unfolding free energy measurements? Level of conformance to common assumptions over an extended range of ribonuclease A stability.

@article{Yao1995HowVA,
  title={How valid are denaturant-induced unfolding free energy measurements? Level of conformance to common assumptions over an extended range of ribonuclease A stability.},
  author={Min Yao and David Wayne Bolen},
  journal={Biochemistry},
  year={1995},
  volume={34 11},
  pages={3771-81}
}
A thermodynamic cycle is used to explore a host of assumptions, conditions, and criteria which must be met for evaluation of authentic unfolding free energy changes. The thermodynamic cycle involves measurement of the unfolding free energy change (delta GzeroN-U) for RNase A at a reference pH, along with determination of the titration free energy changes for the native and unfolded species over an extended pH range. From these free energy changes, delta GzeroN-U at any pH in the range can be… CONTINUE READING

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