How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio RFd of leaves with the PAM fluorometer

@article{Lichtenthaler2005HowTC,
  title={How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio RFd of leaves with the PAM fluorometer},
  author={Hartmut K. Lichtenthaler and C. Buschmann and Martin Knapp},
  journal={Photosynthetica},
  year={2005},
  volume={43},
  pages={379-393}
}
This contribution is a practical guide to the measurement of the different chlorophyll (Chl) fluorescence parameters and gives examples of their development under high-irradiance stress. From the Chl fluorescence induction kinetics upon irradiation of dark-adapted leaves, measured with the PAM fluorometer, various Chl fluorescence parameters, ratios, and quenching coefficients can be determined, which provide information on the functionality of the photosystem 2 (PS2) and the photosynthetic… Expand

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References

SHOWING 1-10 OF 51 REFERENCES
Chlorophyll Fluorescence Parameters: The Definitions, Photosynthetic Meaning, and Mutual Relationships
Chlorophyll fluorescence parameters (Chl FPs) derived from the slow (long-term) induction kinetics of modulated Chl a fluorescence are reviewed and analysed with respect to their application inExpand
Resolution of the Photosystem I and Photosystem II contributions to chlorophyll fluorescence of intact leaves at room temperature.
TLDR
It is shown that the different shapes of these spectra were mainly due to a higher contribution of PSI chlorophylls in the F(O) spectrum, and true value of the relative variable fluorescence of PSII was underestimated in previous works. Expand
Light-induced and Age-dependent Development ofChloroplasts in Etiolated Barley Leaves as Visualized by Determination of Photosynthetic Pigments, C02 Assimilation Rates and Different Kinds of Chlorophyll Fluorescence Ratios
TLDR
The results indicated that the photosynthetic quantum conversion and electron transport within the leaf proceeded at closed stomata and much earlier than measurable CO 2 fixation, apparently ently using internal respiratory CO 2 for photosynthesis. Expand
The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence
Measurements of the quantum yields of chlorophyll fluorescence and CO2 assimilation for a number of plant species exposed to changing light intensity and atmospheric CO2 concentrations and duringExpand
Estimating the contribution of Photosystem I to total leaf chlorophyll fluorescence
Chlorophyll a fluorescence characteristics were investigated in 12 species and 2 hybrids from the genus Flaveria exhibiting C3, C3–C4 intermediate, or C4 photosynthesis, and in the C4 species ZeaExpand
Variation of the Quenching of Chlorophyll Fluorescence under Different Intensities of the Actinic Light in Wildtype Plants of Tobacco and in an Aurea Mutant Deficient of LightHarvesting-Complex
Summary Photochemical quenching, non-photochemical quenching, ratio of fluorescence decrease and several other chlorophyll fluorescence parameters have been studied for two types of tobacco (Expand
The Measurement of Rfd-Values as Plant Vitality Indices with the Portable Field Chlorophyll Fluorometer and the Pam-Fluorometer
The chlorophyll fluorescence vitality indices of green leaves (the Rfd-values) were comparatively determined from the same leaf samples using 1) a self-constructed, red laser equipped, portable fieldExpand
Chlorophyll fluorescence imaging of photosynthetic activity with the flash-lamp fluorescence imaging system
A flash-lamp chlorophyll (Chl) fluorescence imaging system (FL-FIS) is described that allows to screen and image the photosynthetic activity of several thousand leaf points (pixels) of intact leavesExpand
Energy-dependent quenching of dark-level chlorophyll fluorescence in intact leaves
TLDR
A new type of modulation fluorometer was used in the study of energy-dependent chlorophyll fluorescence quenching (qE) in intact leaves, and qE was found to consist of two components, the second of which is linearly correlated with qO. Expand
PHOTOSYNTHESIS AND HIGH LIGHT STRESS
TLDR
In soybean leaves a photoinhibition of the chloroplasts, as measured at the adaxial light exposed upper leaf side, did not affect the photosynthetic CO 2 assimilation rates of the whole leaf, so chlorophyll fluorescence measurements, usually carried out with low irradiance light, taken at the upper leaf-side alone are not representative for the physiological situation of the entire leaf. Expand
...
1
2
3
4
5
...