How the confocal laser scanning microscope entered biological research.

@article{Amos2003HowTC,
  title={How the confocal laser scanning microscope entered biological research.},
  author={William Amos and Jackie G. White},
  journal={Biology of the cell},
  year={2003},
  volume={95 6},
  pages={335-42}
}
A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rather than vice-versa and a high magnification at the level of the detector, allowing the use of a macroscopic iris. These were followed by an achromatic all-reflective relay system, a… CONTINUE READING
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