How the Confocal Laser Scanning Microscope entered Biological Research

@article{Amos2003HowTC,
  title={How the Confocal Laser Scanning Microscope entered Biological Research},
  author={William Bradshaw Amos and J. G. White},
  journal={Biology of the Cell},
  year={2003},
  volume={95}
}
Modern Laser Scanning Confocal Microscopy
TLDR
The principles of confocal microscopy are introduced and how the approach has become a mainstay in the biological sciences are discussed and how modern implementations of the approach have further expanded the use of the technique.
Scanning and Image Reconstruction Techniques in Confocal Laser Scanning Microscopy
Taking advantage of the laser scanning methodology, confocal microscopy has been widely applied in cutting-edge biological research for its three-dimensional, high-resolution imaging capability
Confocal microscopy: from fundamental optics to innovative applications
TLDR
This chapter aims to highlight, to the newcomer to this area, the potential of confocal microscopy to eliminate out of focus planes and thus the blurring of the specimens observed, emphasizing the ability of this method to perform three-dimensional reconstructions, thus generating a new area in 3D microscopy.
Non-scanning Fluorescence Confocal Microscopy using Laser Speckle Illumination
Confocal scanning microscopy (CSM) is a much used and advantageous form of microscopy. Although CSM is superior to conventional microscopy in many respects, a major disadvantage is the complexity of
Modern Confocal Microscopy
TLDR
This unit re‐examines confocal microscopy from a current perspective and introduces programmable array microscopes (PAMs), which are based upon a technique in which spatial modulators are placed in the imaging plane of a microscope and used to generate patterns of illumination and/or detection.
Laser scanning confocal microscopy: history, applications, and related optical sectioning techniques.
TLDR
Significant improvements have been made to all areas of the confocal approach, not only to the instruments themselves, but also to the protocols of specimen preparation, to the analysis, the display, the reproduction, sharing and management of confocal images using bioinformatics techniques.
The use of laser scanning confocal microscopy (LSCM) in materials science
TLDR
This paper provides several examples that exploit the laser scanning confocal microscope's capabilities of pseudo‐infinite depth of field imaging, topographic imaging, photo‐stimulated luminescence imaging and Raman spectroscopic imaging.
Confocal Laser-scanning Microscopy in Filamentous Fungi
TLDR
This chapter focuses primarily on confocal microscopy used to image fungal cells, an advanced fluorescence microscopy technique that utilizes laser illumination, optical devices, pinholes, computers, and cameras/detectors to perform cell and subcellular visualization.
Current opportunities and prospects of confocal laser scanning microscopy in morphological research (review)
TLDR
The study of modern scientific literature has shown the importance of using confocal laser scanning microscopy in modern scientific research and diagnosis of diseases in a clinical setting, which will allow to take a new look at some aspects in modern morphology and medicine.
Modern Trends in Imaging XII Advanced methods in fluorescence microscopy
It requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years. Our effort has been to survey the
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 31 REFERENCES
Optical fluorescence microscopy in three dimensions: microtomoscopy
A unique property of confocal fluorescence microscopy is utilized to obtain scanned images of a number of samples in three dimensions. This kind of microscopy for the first time enables the direct
A standard video-rate confocal laser-scanning reflection and fluorescence microscope
A confocal laser‐scanning microscope (CLSM) differs from a conventional microscope by affording an extreme depth discrimination, as well as a slightly improved resolution. These features afford
An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy
TLDR
It is found that confocal imaging gives greatly enhanced images of biological structures viewed with epifluorescence, and the improvements are such that it is possible to optically section thick specimens with little degradation in the image quality of interior sections.
Confocal scanning light microscopy with high aperture immersion lenses
The imaging characteristics of a confocal scanning light microscope (CSLM) with high aperture, immersion type, lenses (N.A. = 1·3) are investigated. In the confocal arrangement the images of the
Three-dimensional microscopy using a confocal laser scanning microscope.
In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen.
A confocal video‐rate laser‐beam scanning reflected‐light microscope with no moving parts
TLDR
A no‐moving‐parts, 30 frames/s, laser‐beam scanning confocal reflected‐light microscope has been developed and real‐time jitter‐free reflected light images of a variety of biological objects have been produced.
The Origins of Modern Fluorescence Microscopy and Fluorescent Probes
Scanning Optical Microscopy via a Scanning Electron Microscope
We have described in detail the principles of operation of a commercially available SEM-based scanning optical microscope. The stage consists of a cathodoluminescent material which converts the
Two-photon laser scanning fluorescence microscopy.
TLDR
The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Three-dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy
TLDR
The exceptionally short depth of field of this imaging technique provides direct optical sectioning which, together with its higher resolution, makes CSLM extremely useful for studying the three-dimensional morphology of biological structures17–19.
...
1
2
3
4
...