How mutations in tRNA distant from the anticodon affect the fidelity of decoding

@inproceedings{Schmeing2011HowMI,
  title={How mutations in tRNA distant from the anticodon affect the fidelity of decoding},
  author={T. Martin Schmeing and Rebecca M. Voorhees and Ann C. Kelley and V Ramakrishnan},
  booktitle={Nature Structural &Molecular Biology},
  year={2011}
}
The ribosome converts genetic information into protein by selecting aminoacyl tRNAs whose anticodons base-pair to an mRNA codon. Mutations in the tRNA body can perturb this process and affect fidelity. The Hirsh suppressor is a well-studied tRNATrp harboring a G24A mutation that allows readthrough of UGA stop codons. Here we present crystal structures of the 70S ribosome complexed with EF-Tu and aminoacyl tRNA (native tRNATrp, G24A tRNATrp or the miscoding A9C tRNATrp) bound to cognate UGG or… CONTINUE READING