How much effort is required to isolate nuclear microsatellites from plants?

  title={How much effort is required to isolate nuclear microsatellites from plants?},
  author={J. Squirrell and Peter M Hollingsworth and Mary Woodhead and Joanne Russell and A Lowe and M. H. Gibby and Wayne Powell},
  journal={Molecular ecology},
  volume={12 6},
The attributes of codominance, reproducibility and high resolution have all contributed towards the current popularity of nuclear microsatellites as genetic markers in molecular ecological studies. One of their major drawbacks, however, is the development phase required to obtain working primers for a given study species. To facilitate project planning, we have reviewed the literature to quantify the workload involved in isolating nuclear microsatellites from plants. We highlight the attrition… CONTINUE READING
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