How moist heat kills spores of Bacillus subtilis.

  title={How moist heat kills spores of Bacillus subtilis.},
  author={William H. Coleman and De Chen and Yong-qing Li and Ann E. Cowan and Peter Setlow},
  journal={Journal of bacteriology},
  volume={189 23},
Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores that had lost their dipicolinic acid (DPA), undergone significant protein denaturation, and were all dead and a fraction with the same higher density as that of unheated spores. The latter fraction from heat-killed spore populations retained all of its DPA, but >/=98% of the… CONTINUE READING


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