How microtubules get fluorescent speckles.

  title={How microtubules get fluorescent speckles.},
  author={Clare M. Waterman-Storer and Edward D. Salmon},
  journal={Biophysical journal},
  volume={75 4},
The dynamics of microtubules in living cells can be seen by fluorescence microscopy when fluorescently labeled tubulin is microinjected into cells, mixing with the cellular tubulin pool and incorporating into microtubules. The subsequent fluorescence distribution along microtubules can appear "speckled" in high-resolution images obtained with a cooled CCD camera (Waterman-Storer and Salmon, 1997. J. Cell Biol. 139:417-434). In this paper we investigate the origins of these fluorescent speckles… CONTINUE READING

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