How many is enough? exploring the myosin repertoire in the model eukaryoteDictyostelium discoideum

@article{Soldati2007HowMI,
  title={How many is enough? exploring the myosin repertoire in the model eukaryoteDictyostelium discoideum},
  author={Thierry Soldati and Heidrun Geissler and Eva C. Schwarz},
  journal={Cell Biochemistry and Biophysics},
  year={2007},
  volume={30},
  pages={389-411}
}
The cytoplasm of eukaryotic cells is a very complex milieu and unraveling how its unique cytoarchitecture is achieved and maintained is a central theme in modern cell biology. It is crucial to understand how organelles and macro-complexes of RNA and/or proteins are transported to and/or maintained at their specific cellular locations. The importance of filamentous-actindirected myosin-powered cargo transport was only recently realized, and after an initial explosion in the identification of new… Expand
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References

SHOWING 1-10 OF 113 REFERENCES
Examination of the endosomal and lysosomal pathways in Dictyostelium discoideum myosin I mutants.
TLDR
Results suggest that these myosins do not play a role in the intracellular movement of vesicles, but that they may participate in controlling events that occur at the actin-rich cortical region of the cell. Expand
Transport of cytoplasmic particles catalysed by an unconventional myosin in living Drosophila embryos
TLDR
Evidence that transport of cytoplasmic particles in live Drosophila embryos in three dimensions is observed using computational optical sectioning microscopy, the first direct observation of transport catalysed by an unconventional myosin in living cells. Expand
Multiple actin-based motor genes in Dictyostelium.
TLDR
The Dictyostelium genome was probed at low stringency with a gene fragment containing the conserved conventional myosin head domain sequences to identify other actin-based motors that may play a role in the observed motility of these mutant cells. Expand
Myosin I is located at the leading edges of locomoting Dictyostelium amoebae
TLDR
It is suggested that actomyosin I may contribute to the forces that cause extension at the leading edge of a motile cell, while the contraction of actomyOSin II at the rear squeezes the cell mass forward. Expand
Myosin Va associates with microtubule-rich domains in both interphase and dividing cells.
TLDR
Using antibodies that are specific for the Va isoform and immunofluorescence microscopy, it is shown that myosin Va localizes to the microtubule organizing center (MTOC) in interphase cells, and to the mitotic asters, spindle, and midbody of dividing cells. Expand
Dictyostelium discoideum myoJ: a member of a broadly defined myosin V class or a class XI unconventional myosin?
TLDR
Analysis of the available deduced amino acid sequence of the myoJ gene reveals that it possesses six IQ motifs and sequences typical of alpha helical coiled coils in the tail region, and it is predicted to exist as a dimer with up to 12 associated light chains (six per heavy chain). Expand
The 95F unconventional myosin is required for proper organization of the Drosophila syncytial blastoderm
TLDR
It is proposed that 95F myosin function is required to generate normal actin-based transient membrane furrows that separate adjacent mitoses, since microtubule interactions lead to aberrant nuclear divisions and to the nuclear defects observed. Expand
Myosin I Overexpression Impairs Cell Migration
TLDR
Excess full-length cortical myoB prevents the formation of the actin-filled extensions required for locomotion by increasing the tension of the F-actin cytoskeleton and/ or retracting projections before they can fully extend. Expand
The GPQ-rich segment of Dictyostelium myosin IB contains an actin binding site.
TLDR
It is determined that the carboxy-terminal portion of the tail of Dictyostelium myosin IB can bind to actin in an ATP-independent manner and that the actin binding site is contained within residues 922-1059, corresponding to the GPA-rich segment of Acanthamoeba myosIn IC. Expand
Discovery of myosin genes by physical mapping in Dictyostelium.
  • M. Titus, A. Kuspa, W. Loomis
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1994
The diversity of the myosin family in a single organism, Dictyostelium discoideum, has been investigated by a strategy devised to rapidly identify and clone additional members of a gene family. AnExpand
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