How honey kills bacteria

  title={How honey kills bacteria},
  author={Paulus H. S. Kwakman and Anje A. te Velde and Leonie de Boer and Dave Speijer and M. J. Christina Vandenbroucke‐Grauls and Sebastian A. J. Zaat},
  journal={The FASEB Journal},
  pages={2576 - 2582}
With the rise in prevalence of antibiotic-resistant bacteria, honey is increasingly valued for its antibacterial activity. [] Key Result All bacteria tested, including Bacillus subtilis, methicillin-resistant Staphylococcus aureus, extended-spectrum beta-lactamase producing Escherichia coli, ciprofloxacin-resistant Pseudomonas aeruginosa, and vancomycin-resistant Enterococcus faecium, were killed by 10-20% (v/v) honey, whereas > or = 40% (v/v) of a honey-equivalent sugar solution was required for similar…

Medical-grade honey enriched with antimicrobial peptides has enhanced activity against antibiotic-resistant pathogens

It was not feasible to enhance the rapid activity of RS honey by enrichment with endogenous compounds, but RS honey enriched with 75 μM of the synthetic peptide Bactericidal Peptide 2 (BP2) showed rapid bactericidal activity against all species tested, including MRSA and ESBL E. coli, at up to 10–20-fold dilution.

Two Major Medicinal Honeys Have Different Mechanisms of Bactericidal Activity

Honey is increasingly valued for its antibacterial activity, but knowledge regarding the mechanism of action is still incomplete, resulting in large differences in bactericidal activity of RS and manuka honey.

Manuka honey inhibits cell division in methicillin-resistant Staphylococcus aureus.

Enlarged cells containing septa were observed in MRSA exposed to inhibitory concentrations of manuka honey, suggesting that cell division was interrupted and indicate the presence of additional antibacterial components in manuka Honey.

Manuka-type honeys can eradicate biofilms produced by Staphylococcus aureus strains with different biofilm-forming abilities

New Zealand manuka-type honeys, at the concentrations they can be applied in wound dressings are highly active in both preventing S. aureus biofilm formation and in their eradication, and do not result in bacteria becoming resistant.

Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa.

Ulmo 90 honey, a Chilean honey made by Apis mellifera originating from the Ulmo tree, has high antimicrobial activity and may warrant further investigation as a possible alternative therapy for wound healing.

Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa

The results indicate that methylglyoxal inhibits the growth of MDRP at concentrations of 128–512 μg/ml (1.7–7.1 mM) and is not recognized by drug efflux systems.

In vitro antibacterial activity of honey against Staphylococcus aureus and Escherichia coli

The in-vitro antibacterial effectiveness of different types of honey was tested against two species of bacteria; Staphylococcus aureus and Escherichia coli, using the disk diffusion method to point to the potential use of honey as an antibacterial agent and therefore a possible alternative therapy against ailments caused by these two bacterial species.

Antimicrobial Evaluation of Various Honey Types against Carbapenemase-Producing Gram-Negative Clinical Isolates

The in vitro antibacterial potential of rare Greek honeys against Verona integron-encoded metallo-β-lactamase (VIM)- or Klebsiella pneumoniae carbapenemase-producing multidrug-resistant Gram-negative pathogens is studied.

Antimicrobial Activity of Different Honey Types Against Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa and Candida albicans

The eight types of Libyan honey have a various antibacterial activity in-vitro, according to the zone of inhibition against each type of microorganism.



Medical-grade honey kills antibiotic-resistant bacteria in vitro and eradicates skin colonization.

Revamil is a promising topical antimicrobial agent for prevention or treatment of infections, including those caused by multidrug-resistant bacteria.

Inhibitory activity of monofloral and multifloral honeys against bacterial pathogens

Honey samples evaluated for their ability to inhibit the growth of ten bacterial pathogens exhibited different sensitivities to honeys, and S.sonnei was the pathogen whose growth was most inhibited by honey.

Honey: A Potent Agent for Wound Healing?

  • P. LusbyA. CoombesJ. Wilkinson
  • Medicine
    Journal of wound, ostomy, and continence nursing : official publication of The Wound, Ostomy and Continence Nurses Society
  • 2002
What is known about the medical properties of honey is outlined and the potential for honey to be incorporated into the management of a large number of wound types is indicated.

Involvement of bactericidal factors from thrombin-stimulated platelets in clearance of adherent viridans streptococci in experimental infective endocarditis

In vitro studies with VGs excised 5 min after challenge with stain 1 or 2 showed that clearance of the releasate-susceptible strain 1 was not caused by complement bactericidal activity or surface phagocytosis by polymorphonuclear cells, and the final developments of IE were not significantly different.

Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity.

Overall, these studies show that HNP-mediated bactericidal activity against E. coli ML-35 is associated with sequential permeabilization of the OM and IM, and that inner membrane permeabilizations appears to be the lethal event.

Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand.

For six samples of New Zealand Manuka (Leptospermum scoparium) honey very high amounts of MGO were found, ranging from 38 to 761 mg/kg, which is up to 100-fold higher compared to conventional honeys, which clearly demonstrates that the pronounced antibacterial activity of NewNZ Manuka honey directly originates from MGO.

Ultrasensitive assays for endogenous antimicrobial polypeptides.

Expression of amylase and glucose oxidase in the hypopharyngeal gland with an age-dependent role change of the worker honeybee (Apis mellifera L.).

Results clearly indicate that expression of the genes for these carbohydrate-metabolizing enzymes, which are needed to process nectar into honey, in the hypopharyngeal gland is associated with the age-dependent role change of the worker bees.