How enzymes adapt: lessons from directed evolution.

  title={How enzymes adapt: lessons from directed evolution.},
  author={Frances H. Arnold and Patrick L. Wintrode and Kentaro Miyazaki and Alexander Gershenson},
  journal={Trends in biochemical sciences},
  volume={26 2},
Enzymes that are adapted to widely different temperature niches are being used to investigate the molecular basis of protein stability and enzyme function. However, natural evolution is complex: random noise, historical accidents and ignorance of the selection pressures at work during adaptation all cloud comparative studies. Here, we review how adaptation in the laboratory by directed evolution can complement studies of natural enzymes in the effort to understand stability and function… CONTINUE READING

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