How crowded is the cytoplasm?

@article{Fulton1982HowCI,
  title={How crowded is the cytoplasm?},
  author={Alice Bordwell Fulton},
  journal={Cell},
  year={1982},
  volume={30},
  pages={345-347}
}
Physiological Environment Induces Quick Response – Slow Exhaustion Reactions
TLDR
The spectral dimension (ds), which can be translated into the reaction rate function, suggests that in vivo reactions initially run faster than the same reactions in a homogeneous space; this conclusion is consistent with the anomalous character indicated by FCS analysis. Expand
Evolutionary drivers of protein shape
TLDR
The dynamics of triaxial ellipsoids of equivalent steric volume to proteins at different aspect ratios and volume fractions are studied using a combination of Brownian molecular dynamics and geometric packing to find proteins to have a shape that gives rise to the highest critical volume fraction resisting gelation. Expand
EFFECT OF HYDRATION AND MACROMOLECULAR CROWDING ON PEPTIDE CONFORMATION, AGGREGATION AND FOLDING KINETICS
Protein folding/misfolding in vivo takes place in a highly crowded and confined environment. Such crowded environment can possibly lead to fewer water molecules surrounding a protein of interest thanExpand
Quantitative characterization of temperature-independent polymer–polymer interaction and temperature-dependent protein–protein and protein–polymer interactions in concentrated polymer solutions
TLDR
Dextran T70 contributes more to the attractive protein–polymer interaction and to self-association of protein than Ficoll 70 and reasons for this are discussed. Expand
Single-cell proteomics reveals downregulation of TMSB4X to drive actin release for stereocilia assembly
TLDR
It is proposed that mostActin is sequestered by TMSB4X in progenitor cells, but upon differentiation to hair cells, actin is released to build the sensory hair bundle. Expand
Life in a crowded world Workshop on the Biological Implications of Macromolecular Crowding
Introduction Macromolecules are present as soluble species and/or structural arrays at total concentrations of up to several hundred grams per litre in essentially all physiological compartments.Expand
Evidence for a pathway that facilitates nitric oxide diffusion in the brain
TLDR
It is indicated that neither ()NO diffusion through the brain extracellular space nor homogeneous diffusion in the tissue through brain cells can account for the similarity between ()NO free diffusion coefficient and that obtained in the brain. Expand
Metabolic Compartmentation – A System Level Property of Muscle Cells
TLDR
It can be concluded that local restrictions of diffusion of metabolites in a cell are a system-level properties caused by complex structural organization of the cells, macromolecular crowding, cytoskeletal networks and organization of metabolic pathways into multienzyme complexes and metabolons. Expand
Swelling of hepatocytes injured by oxidative stress suggests pathological changes related to macromolecular crowding
A fundamental property of living matter is the ability to establish and maintain order. Mild changes in cell volume have a role in metabolic control. Furthermore, cellular swelling is a way of livingExpand
Life in a crowded world
TLDR
Experimental and theoretical work has demonstrated substantial (order‐of‐magnitude) effects of crowding on a broad range of biochemical, biophysical and physiological processes, including—but not limited to—nucleic acid and protein conformation and stability, protein–protein and protein–DNA association equilibria and kinetics. Expand
...
1
2
3
4
5
...