How complement kills E. coli. II. The apparent two-hit nature of the lethal event.

@article{Wright1981HowCK,
  title={How complement kills E. coli. II. The apparent two-hit nature of the lethal event.},
  author={Samuel D. Wright and Robert Paul Levine},
  journal={Journal of immunology},
  year={1981},
  volume={127 3},
  pages={1152-6}
}
We have studied the nature of complement (C) action on red blood cells and E. coli with respect to the number of "hits" required for membrane damage. Our method of analysis involves adding various amounts of purified C7 or C8 to serum preparations immunochemically depleted of C7 or C8, respectively, in order to construct dose-response curves for the action of C's terminal complex. The shape of the dose-response curves reflects the single or multiple-hit nature of C action. Our method confirms… CONTINUE READING