How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing.

@article{James2002HowSA,
  title={How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing.},
  author={Shelly-Ann James and William R. Turner and Beate Schwer},
  journal={RNA},
  year={2002},
  volume={8 8},
  pages={1068-77}
}
Slu7 and Prp18 act in concert during the second step of yeast pre-mRNA splicing. Here we show that the 382-amino-acid Slu7 protein contains two functionally important domains: a zinc knuckle (122CRNCGEAGHKEKDC135) and a Prp18-interaction domain (215EIELMKLELY224). Alanine cluster mutations of 215EIE217 and 221LELY224 abrogated Slu7 binding to Prp18 in a two-hybrid assay and in vitro, and elicited temperature-sensitive growth phenotypes in vivo. Yet, the mutations had no impact on Slu7 function… CONTINUE READING

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