Hot spots of DNA instability revealed through the study of somaclonal variation in rye

@article{Linacero2000HotSO,
  title={Hot spots of DNA instability revealed through the study of somaclonal variation in rye},
  author={Rosario Linacero and Elo{\'a} Muniz de Freitas Alves and Ana Mar{\'i}a V{\'a}zquez},
  journal={Theoretical and Applied Genetics},
  year={2000},
  volume={100},
  pages={506-511}
}
RAPD analysis was performed to assess DNA variation among rye plants regenerated from immature embryos and inflorescences. From the studied plants, 40% showed at least one variation, and the number of mutations per plant was quite high, ranging from 1 up to 12. On some occasions (2.9% of the scored bands) the modified band was observed in only one plant or in several but originated from the same callus (variable band). In other cases (5.25%) the same band varied in several plants obtained from… CONTINUE READING
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