Host-vector system for integration of recombinant DNA into chromosomes of transformable and nontransformable streptococci.

@article{Pozzi1988HostvectorSF,
  title={Host-vector system for integration of recombinant DNA into chromosomes of transformable and nontransformable streptococci.},
  author={Gianni Pozzi and Rosa Anna Musmanno and Elisabetta A Renzoni and Marco R Oggioni and Maria Grazia Cusi},
  journal={Journal of bacteriology},
  year={1988},
  volume={170 4},
  pages={1969-72}
}
We describe a genetic system in which transformation of Streptococcus pneumoniae and Streptococcus sanguis was used to insert recombinant DNA into the conjugative chromosomal element omega (cat tetM) 6001 (omega 6001). The element containing the recombinant DNA was then transferred by conjugation to the chromosome of transformable and nontransformable streptococci. When Escherichia coli plasmid pDP36 was used as donor in transformation, it was capable of inserting 5.9 kilobases of heterologous… CONTINUE READING
Highly Cited
This paper has 102 citations. REVIEW CITATIONS
10 Citations
12 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

102 Citations

0204060'97'02'08'14
Citations per Year
Semantic Scholar estimates that this publication has 102 citations based on the available data.

See our FAQ for additional information.

References

Publications referenced by this paper.
Showing 1-10 of 12 references

Alkaline Phophatase inhibits cloning in Streptococcus pneumoniae

  • G. Pozzi, W. R. Guild
  • FEMS Microbiol. Lett. 41:309-311
  • 1987

Conjugative transfer of chromosomal R determinants in Streptococcus pneumoniae, p. 88-92

  • W. R. Guild, M. D. Smith, N. B. Shoemaker
  • In D. Shlessinger (ed.),
  • 1982

Similar Papers

Loading similar papers…