Host-dependent roles of the viral 5' untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3' UTR of Red clover necrotic mosaic virus RNA1.

@article{Sarawaneeyaruk2009HostdependentRO,
  title={Host-dependent roles of the viral 5' untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3' UTR of Red clover necrotic mosaic virus RNA1.},
  author={Siriruk Sarawaneeyaruk and Hiro-oki Iwakawa and Hiroyuki Mizumoto and Hiromi Murakami and Masanori Kaido and Kazuyuki Mise and Tetsuro Okuno},
  journal={Virology},
  year={2009},
  volume={391 1},
  pages={
          107-18
        }
}
The genome of Red clover necrotic mosaic virus (RCNMV) consists of RNA1 and RNA2, both lacking a cap structure and a poly(A)tail. RNA1 has a translational enhancer element (3'TE-DR1) in the 3' untranslated region (UTR). In this study, we analyzed the roles of 5' and 3' UTRs of RNA1 in 3'TE-DR1-mediated cap-independent translation in cowpea and tobacco BY-2 protoplasts using a dual-luciferase (Luc) reporter assay system. Most mutations introduced into RNA1 5' UTR in reporter Luc mRNA abolished… CONTINUE READING

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