Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification.

@article{Levy2016HostCP,
  title={Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification.},
  author={Nicholas E Levy and Kristin N Valente and Kelvin H. Lee and Abraham M Lenhoff},
  journal={Biotechnology and bioengineering},
  year={2016},
  volume={113 6},
  pages={1260-72}
}
Downstream purification of monoclonal antibodies (mAbs) is normally performed using a platform process that is empirically tuned to optimize impurity removal for each new product. A more fundamental understanding of impurities and the product itself would provide insights into the rational design of efficient downstream processes. This work examines the chromatographic properties of Chinese hamster ovary host cell protein (HCP) impurities in non-affinity chromatographic resins commonly used in… CONTINUE READING

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