Host cell protein dynamics in the supernatant of a mAb producing CHO cell line.

@article{Tait2012HostCP,
  title={Host cell protein dynamics in the supernatant of a mAb producing CHO cell line.},
  author={Andrew S. Tait and Catherine E M Hogwood and Christopher Mark Smales and Daniel G Bracewell},
  journal={Biotechnology and bioengineering},
  year={2012},
  volume={109 4},
  pages={971-82}
}
The characterization of host cell protein (HCP) content during the production of therapeutic recombinant proteins is an important aspect in the drug development process. Despite this, key components of the HCP profile and how this changes with processing has not been fully investigated. Here we have investigated the supernatant HCP profile at different times throughout culture of a null and model GS-CHO monoclonal antibody producing mammalian cell line grown in fed-batch mode. Using 2D-PAGE and… CONTINUE READING
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