Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.

@article{Fredrikson1981HormonesensitiveLO,
  title={Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.},
  author={Gudrun Fredrikson and P Str{\aa}lfors and Nils Osten Nilsson and Per Belfrage},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 12},
  pages={6311-20}
}
Hormone-sensitive lipase from rat adipose tissue was solubilized with nonionic detergent and purified 2,000fold to 50% protein purity by repeated gradient sievorptive chromatography on diethyL(2-hydroxypropy1)aminoethyl-Sephadex and by affinity chromatography on triacylglycerol-containing polyacrylamideagarose gel. The identity of the enzyme protein, a polypeptide with M, = 84,000 by polyacrylamide gel electrophoresis in sodium dodecyl sulfate, was established by its copurification with lipase… CONTINUE READING

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