Corpus ID: 32711104

Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.

  title={Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.},
  author={G. Fredrikson and P. Str{\aa}lfors and N. O. Nilsson and P. Belfrage},
  journal={The Journal of biological chemistry},
  volume={256 12},
  • G. Fredrikson, P. Strålfors, +1 author P. Belfrage
  • Published 1981
  • Biology, Medicine
  • The Journal of biological chemistry
  • Hormone-sensitive lipase from rat adipose tissue was solubilized with nonionic detergent and purified 2,000fold to 50% protein purity by repeated gradient sievorptive chromatography on diethyL(2-hydroxypropy1)aminoethyl-Sephadex and by affinity chromatography on triacylglycerol-containing polyacrylamideagarose gel. The identity of the enzyme protein, a polypeptide with M, = 84,000 by polyacrylamide gel electrophoresis in sodium dodecyl sulfate, was established by its copurification with lipase… CONTINUE READING