Hormone-dependent covalent modification and processing of human progesterone receptors in the nucleus.

@article{Horwitz1985HormonedependentCM,
  title={Hormone-dependent covalent modification and processing of human progesterone receptors in the nucleus.},
  author={Kathryn B. Horwitz and Mary D. Francis and Lisa L. Wei},
  journal={DNA},
  year={1985},
  volume={4 6},
  pages={451-60}
}
In situ photoaffinity labeling, which minimizes in vitro incubations and proteolytic artifacts, was used to study the structure of progesterone receptors (PR) in intact T47D human breast cancer cells. These cells, rich in PR, were incubated with the photoreactive progestin [3H]R5020 at 0 degrees C for 3 hr to keep PR in their untransformed state, or at 37 degrees C for 5 min to transform PR and convert them to tight chromatin-binding proteins. The cells, still intact, were then irradiated with… CONTINUE READING

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