Hormone Secretion by Cells Dissociated from Rat Anterior Pituitaries

  title={Hormone Secretion by Cells Dissociated from Rat Anterior Pituitaries},
  author={Colin R. Hopkins and Marilyn Gist Farquhar},
  journal={The Journal of Cell Biology},
  pages={276 - 303}
A new procedure has been developed for dissociating anterior pituitary tissue and producing a viable suspension of single cells. The procedure involves incubation of small tissue blocks in 1 mg/ml trypsin (15 min), followed by incubation in 8 microg/ml neuraminidase and 1 mM EDTA (15 min), followed by mechanical dispersion. Cell yields are approximately 55%, based on recovered DNA. By electron microscopy five types of secretory cells (somatotrophs, mammotrophs, thyrotrophs, gonadotrophs, and… CONTINUE READING
34 Citations
34 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 34 extracted citations


Publications referenced by this paper.
Showing 1-10 of 34 references

Processing of secretory products by cells of the anterior pituitary gland

  • H. Heller and K. Lederis, editors . Mem. Soc…
  • 1971

Bioassay of the hypophysiotrophic hormones : In vitro

  • VALE
  • 1970

Similar Papers

Loading similar papers…