Hormonal regulation of apoptosis in rabbit granulosa cells in vitro: evaluation by flow cytometric detection of plasma membrane phosphatidylserine externalization.

@article{Maillet2002HormonalRO,
  title={Hormonal regulation of apoptosis in rabbit granulosa cells in vitro: evaluation by flow cytometric detection of plasma membrane phosphatidylserine externalization.},
  author={G{\'e}raldine Maillet and Emmanuel Br{\'e}ard and Annie Benha{\"i}m and Pierre Leymarie and Colette F{\'e}ral},
  journal={Reproduction},
  year={2002},
  volume={123 2},
  pages={
          243-51
        }
}
Annexin V and propidium iodide bivariate analysis and the TUNEL method were used to quantify hormonal regulation of apoptosis in rabbit granulosa cells from preovulatory follicles in vitro. The aim of this study was to analyse comparatively the effects of gonadotrophins and their second messenger in the regulation of granulosa cell apoptosis in (i) cultured isolated granulosa cells and (ii) granulosa cells scraped from cultured follicles. The results showed that increasing doses of FSH had no… CONTINUE READING
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