Homotypic Lysosome Fusion in Macrophages: Analysis Using an In Vitro Assay

@article{Ward1997HomotypicLF,
  title={Homotypic Lysosome Fusion in Macrophages: Analysis Using an In Vitro Assay },
  author={Diane M Ward and Jonathan D. Leslie and Jerry Kaplan},
  journal={The Journal of Cell Biology},
  year={1997},
  volume={139},
  pages={665 - 673}
}
Lysosomes are dynamic structures capable of fusing with endosomes as well as other lysosomes. We examined the biochemical requirements for homotypic lysosome fusion in vitro using lysosomes obtained from rabbit alveolar macrophages or the cultured macrophage-like cell line, J774E. The in vitro assay measures the formation of a biotinylated HRP-avidin conjugate, in which biotinylated HRP and avidin were accumulated in lysosomes by receptor-mediated endocytosis. We determined that lysosome fusion… CONTINUE READING