Homology of Saccharomyces cerevisiae ADH4 to an iron-activated alcohol dehydrogenase from Zymomonas mobilis

@article{Williamson2004HomologyOS,
  title={Homology of Saccharomyces cerevisiae ADH4 to an iron-activated alcohol dehydrogenase from Zymomonas mobilis},
  author={V. Williamson and Charlotte E. Paquin},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={209},
  pages={374-381}
}
SummaryInsertion of the transposable element Ty at the ADH4 locus results in increased levels of a new alcohol dehydrogenase (ADH) activity in Saccharomyces cerevisiae. The DNA sequence of this locus has been determined. It contains a long open reading frame which is not homologous to the other ADH isozymes that have been characterized in S. cerevisiae nor does it show obvious homology to Drosophila ADH. The hypothetical ADH does, however, show strong homology to the sequence of an iron… Expand
ADH 2 ( YMR 303 C ) encoding Adh 2
Alcohol dehydrogenases (ADHs) constitute a large family of enzymes responsible for the reversible oxidation of alcohols to aldehydes with the concomitant reduction of NAD or NADP. These enzymes haveExpand
Isolation ofCaenorhabditis elegans mutants lacking alcohol dehydrogenase activity
TLDR
Allyl alcohol-resistant mutant strains were selected from ethylmethanesulfonate (EMS)-mutagenized nematode populations and ADH activity was measured in extracts from eight of these strains and was found to be low or nondetectable. Expand
The alcohol dehydrogenases of Saccharomyces cerevisiae: a comprehensive review.
TLDR
A brief summary of the wealth of knowledge gained from earlier studies as well as more recent work regarding the primary and secondary structure, kinetic characteristics, function and molecular regulation of the ADHs in S. cerevisiae is provided. Expand
A distinct type of alcohol dehydrogenase, adh4+, complements ethanol fermentation in an adh1-deficient strain of Schizosaccharomyces pombe.
TLDR
DNA microarray and RT-PCR analysis demonstrated that this ethanol production is caused by the enhanced expression of a Saccharomyces cerevisiae ADH4-like gene product (SPAC5H10.06C named adh4(+)). Expand
Evolution of the alcohol dehydrogenase (ADH) genes in yeast: characterization of a fourth ADH in Kluyveromyces lactis
TLDR
The results suggest that K. lactis and S. cerevisiae use different compartments for their metabolism of ethanol, and indicate that the complex regulatory circuits controlling the glucose-repressible SADH2 in S. Cerevisiae are a recent acquisition from regulatory networks used for the control of genes other than S ADH2. Expand
Identification of a mitochondrial alcohol dehydrogenase in Schizosaccharomyces pombe: new insights into energy metabolism.
In the present study we have shown that mitochondria isolated from Schizosaccharomyces pombe exhibit antimycin A-sensitive oxygen uptake activity that is exclusively dependent on ethanol and isExpand
Cloning and Characterization of a Novel Human Alcohol Dehydrogenase Gene (ADHFe1)
TLDR
The hypothetical alcohol dehydrogenase does, however, show strong homology to the iron-activatedalcohol dehydrogenases from microbial organisms. Expand
Molecular and physiological aspects of alcohol dehydrogenases in the ethanol metabolism of Saccharomyces cerevisiae.
TLDR
Transcription profiles of the ADH4 and ADH5 genes suggested that participation of these gene products in ethanol production from glucose was unlikely, and cultivation with glucose or ethanol as carbon substrate revealed that Adh1 was the only alcohol dehydrogenase capable of efficiently catalysing the reduction of acetaldehyde to ethanol. Expand
Role of Iron-Containing Alcohol Dehydrogenases in Acinetobacter baumannii ATCC 19606 Stress Resistance and Virulence
TLDR
The results suggest that ADH4 is primarily involved in alcohol metabolism, while ADH3 and ADH6 are key to stress resistance and virulence, and further investigation into the roles of other ADHs in A. baumannii is warranted. Expand
Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1.
TLDR
A NAD+-dependent medium-chain alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1 was expressed in Escherichia coli and purified and maintained 24% of the original catalytic activity after incubation for 30 min at 98 degrees C, indicating that this enzyme is highly thermostable. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 41 REFERENCES
Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae: I. Isolation and genetic analysis of adh mutants
TLDR
Genetic analysis showed that two genes control synthesis of the glucose repressible enzyme ADHII, one gene the constitutive ADHI and a fourth nuclear gene the mitochondrial ADH showed any linkage. Expand
Isolation of the structural gene for alcohol dehydrogenase by genetic complementation in yeast
TLDR
The use of the newly developed technique of yeast transformation to isolate recombinant plasmids that contain the structural gene for ADHI (ADC1) is described, selected from a pool of plasmid containing yeast DNA by virtue of their ability to complement the growth defect of yeast strains completely lacking ADH activity. Expand
Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis
The gene which encodes alcohol dehydrogenase II (adhB) from Zymomonas mobilis was cloned in Escherichia coli as a 1.4-kilobase DNA fragment by using a novel indicator plate which directly detects theExpand
Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II
TLDR
It appears that insertion of a transposable element near the 5' terminus of the structural gene can produce constitutive expression of a normally glucose-repressed enzyme. Expand
The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
TLDR
The amino acid sequence of the protein as determined from the nucleotide sequence disagrees with the published alcohol dehydrogenase isozyme I (ADH-I) sequence for 5 of the 347 amino acid residues. Expand
Isolation and DNA sequence of ADH3, a nuclear gene encoding the mitochondrial isozyme of alcohol dehydrogenase in Saccharomyces cerevisiae.
TLDR
The cloned gene integrated at the ADH3 locus, indicating that this is the structural gene for ADH III, and the nucleotide sequence of the presumed leader peptide has a high degree of identity with the untranslated leader regions of ADH1 and ADH2 mRNAs. Expand
Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. II. Two loci controlling synthesis of the glucose-repressible ADH II.
  • M. Ciriacy
  • Biology, Medicine
  • Molecular & general genetics : MGG
  • 1975
TLDR
It was demonstrated, that allelic interactions at the protein level are not restricted to electrophoretical behaviour of hybrid enzymes and they also influence specific activities and substrate affinities. Expand
Resistance to antimycin A in yeast by amplification of ADH4 on a linear, 42 kb palindromic plasmid
TLDR
A yeast strain lacking a functional copy of ADH1 has been isolated that is resistant to antimycin A because of the presence of multiple copies of a nuclear gene, ADH4, which is the most distal marker on the left arm of chromosome VII. Expand
The complete amino acid sequence of three alcohol dehydrogenase alleloenzymes (AdhN-11, AdhS and AdhUF) from the fruitfly Drosophila melanogaster.
  • D. Thatcher
  • Biology, Medicine
  • The Biochemical journal
  • 1980
The sequence of three alcohol dehydrogenase alleloenzymes from the fruitfly Drosophila melanogaster has been determined by the sequencing of peptides produced by trypsin, chymotrypsin, thermolysin,Expand
DNA sequences of two yeast promoter-up mutants
TLDR
Sequencing of the 5′-flanking regions of two promoter-up, constitutive ADR2 mutants are reported, in both of which the mutant phenotype is associated with an increase in length of a poly(A)·poly(T) tract 222 base pairs (bp) upstream of the gene. Expand
...
1
2
3
4
5
...