Homology-facilitated plasmid transfer in Haemophilus influenzae

  title={Homology-facilitated plasmid transfer in Haemophilus influenzae},
  author={Johan H. Stuy and Ronald B. Walter},
  journal={Molecular and General Genetics MGG},
The 8 kbp plasmid pAT4 transformed Haemophilus influenzae Rd cells at low frequencies. Transformation was increased up to 100 times, however, when the recipient cells carried a DNA segment in either their chromosome or in a resident plasmid that was homologous to at least part of plasmid pAT4. Linearized plasmid DNA molecules did not transform cells without DNA homology; they efficiently transformed homology recipients, but only when the cuts had been made in the region of shared homology. In… CONTINUE READING


Publications referenced by this paper.
Showing 1-10 of 46 references

Similar Papers

Loading similar papers…