Homologues of vertebrate type I, II and III intermediate filament (IF) proteins in an invertebrate: the IF multigene family of the cephalochordate Branchiostoma

@article{Karabinos1998HomologuesOV,
  title={Homologues of vertebrate type I, II and III intermediate filament (IF) proteins in an invertebrate: the IF multigene family of the cephalochordate Branchiostoma},
  author={Anton Karabinos and Dieter Riemer and Andreas Erber and Klaus Weber},
  journal={FEBS Letters},
  year={1998},
  volume={437}
}
We searched for functional homologues of the four subfamilies of vertebrate cytoplasmic intermediate filament (IF) proteins in the cephalochordate Branchiostoma. The epidermis contains in addition to IF proteins C2 and D1 two novel IF proteins E1 and E2. Both sequence comparisons as well as the obligatory heteropolymer formation by the recombinant proteins identify E1 as a type I keratin and E2 and D1 as type II keratins. In contrast the non‐epidermal B1 forms as type III homologue… Expand
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22 Citations
Background Citations
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Methods Citations
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Results Citations
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22 Citations

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Citation Type

Characterisation and tissue-specific expression of the two keratin subfamilies of intermediate filament proteins in the cephalochordate Branchiostoma.
TLDR
The cloning of three intermediate filament proteins expressed at the gastrula stage (kl, Y1, X1) extends the size of the IF multigene family of Branchiostoma to at least 13 members, and is discussed whether the remaining 8 IF proteins reflect three additional and potentially cephalochordate-specific subfamilies. Expand
Tissue-specific co-expression and in vitro heteropolymer formation of the two small branchiostoma intermediate filament proteins A3 and B2.
The two small intermediate filament (IF) proteins A3 and B2 of the cephalochordate Amphioxus were investigated. Blot overlays indicated a heterotypic interaction pattern of the recombinant proteins.… Expand
Tissue-Specific Co-expression and in vitro Heteropolymer Formation of the Two Small Branchiostoma Intermediate Filament Proteins A3 and B2
The two small intermediate filament (IF) proteins A3 and B2 of the cephalochordate Amphioxus were investigated. Blot overlays indicated a heterotypic interaction pattern of the recombinant proteins.… Expand
The cephalochordate Branchiostoma genome contains 26 intermediate filament (IF) genes: implications for evolution of chordate IF proteins.
  • A. Karabinos
  • Biology, Medicine
  • European journal of cell biology
  • 2013
TLDR
A few type I and type II keratin genes existed in a common ancestor of cephalochordate Branchiostoma floridae and vertebrates, which after separation of these two lineages gave rise to the known complexities of the vertebrate cytoplasmic type I-IV IF proteins, as well as to the multiple keratin and related IF genes in cep Khalochordates, due to multiple gene duplications, deletions and sequence divergences. Expand
The epidermal intermediate filament proteins of tunicates are distant keratins; a polymerisation-competent hetero coiled coil of the Styela D protein and Xenopus keratin 8.
TLDR
It is proposed that Styela D retains, in spite of its strong sequence drift, important molecular features of type I keratins, and it is discussed that type I to III IF proteins are expressed along the chordate branch of metazoa. Expand
Genes coding for intermediate filament proteins closely related to the hagfish “thread keratins (TK)” α and γ also exist in lamprey, teleosts and amphibians
TLDR
Five novel IF proteins closely related to TKα and TKγ, respectively are sequenced from lamprey, and it seems likely that the keratin gene(s) originated from an ancient type III IF protein gene. Expand
Intermediate filament (IF) proteins IFA-1 and IFB-1 represent a basic heteropolymeric IF cytoskeleton of nematodes: A molecular phylogeny of nematode IFs.
TLDR
Phylogenetic analyses suggest that all nematode IFs might arose from a AB-type IF ancestor through repeated gene duplications and sequence divergence, and Interestingly, the C. elegans IF proteins IFA-1 and IFB-1 represent a heteropolymeric IF cytoskeleton in all investigated nematodes clades, in contrast to other sequences restricted to the clade III-V. Expand
Developmentally controlled expression patterns of intermediate filament proteins in the cephalochordate Branchiostoma
TLDR
Two-dimensional gels confirm that keratins are the major IF proteins in the nerve cord and Immunogold electronmicroscopy shows that proteins X1 and C2 are present in epidermis and nerve cord in keratin IF. Expand
Evolutionary aspects in intermediate filament proteins.
TLDR
Proteins of the IF family fulfil increasingly diverse functions but share common structural features and major steps in lamin evolution occurred at the base of the vertebrate radiation, while cytoplasmic IF protein subfamilies evolved independently in the major metazoan lineages. Expand
A rod domain sequence in segment 1B triggers dimerisation of the two small Branchiostoma IF proteins B2 and A3.
TLDR
A part of the B2 sequence is identified which retains the ability of the full length protein B2 to specifically recognize A3 in blot overlays and shares with the currently known trigger-like motif of cortexillin and other coiled coil proteins potential to form multiple inter-chain ionic interactions. Expand
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