Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.

@article{Kucherlapati1984HomologousRB,
  title={Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.},
  author={Raju S. Kucherlapati and Eva M. Eves and Kye Yong Song and Bernard S. Morse and O. Smithies},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 10},
  pages={3153-7}
}
We have used the eukaryotic-prokaryotic shuttle vector pSV2Neo to demonstrate that cultured mammalian somatic cells have the enzymatic machinery to mediate homologous recombination and that the frequency of this recombination can be enhanced by pretreatment of the input DNA. Two nonoverlapping deletion mutants of pSV2Neo were constructed, each affecting the bacterial aminoglycoside 3'-phosphorylase gene (the neo gene), which confers resistance to aminoglycoside antibiotics on bacteria and… CONTINUE READING

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