Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli.

@article{Bryant2017HomologousRT,
  title={Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli.},
  author={Jack A. Bryant and David J. Lee},
  journal={Methods in molecular biology},
  year={2017},
  volume={1624},
  pages={
          3-16
        }
}
Homologous recombination methods enable modifications to be made to the bacterial chromosome. Commonly, the λ phage RED proteins are employed as a site-specific recombinase system, to facilitate recombination of linear DNA fragments with targeted regions of the chromosome. Here we describe methods for the efficient delivery of linear DNA segments containing… CONTINUE READING