Homoisocitric dehydrogenase from yeast.

@article{Rowley1970HomoisocitricDF,
  title={Homoisocitric dehydrogenase from yeast.},
  author={B. Rowley and A. Tucci},
  journal={Archives of biochemistry and biophysics},
  year={1970},
  volume={141 2},
  pages={
          499-510
        }
}
  • B. Rowley, A. Tucci
  • Published 1970
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
Abstract Homoisocitric dehydrogenase, which catalyzes the reversible transformation of homoisocitrate (1-hydroxy-1,2,4-butanetricarboxylic acid) to α-ketoadipate, has been purified approximately 500-fold from extracts of baker's yeast by protamine sulfate treatment, ammonium sulfate fractionation, heat and pH 4 precipitation of contaminants, and chromatography on a column of hydroxylapatite mixed with cellulose. The molecular weight of the enzyme was 48,000 as determined by gel filtration… Expand

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