Homogeneous immunoassays using rare earth cryptates and time resolved fluorescence: principles and specific advantages for tumor markers.

@article{Mathis1997HomogeneousIU,
  title={Homogeneous immunoassays using rare earth cryptates and time resolved fluorescence: principles and specific advantages for tumor markers.},
  author={G{\'e}rard Mathis and F Socquet and M Viguier and Bruno Darbouret},
  journal={Anticancer research},
  year={1997},
  volume={17 4B},
  pages={3011-4}
}
We have developed a new type of long lived fluorescent tracer, the rare earth cryptates formed by the inclusion of a Eu3+ ion into the intra molecular cavity of a macrobicyclic ligand containing bipyridine units. This tracer is used in a new homogeneous assay format based on spectral and temporal selectivity as well as on an amplification of the cryptate fluorescence. As a consequence of these features, the measurement of the specific signal is totally shielded from media interaction and… CONTINUE READING