Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin.

@article{Nakashima2006HomogeneousER,
  title={Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin.},
  author={Kazunori Nakashima and Tatsuo Maruyama and Noriho Kamiya and Masahiro Goto},
  journal={Organic & biomolecular chemistry},
  year={2006},
  volume={4 18},
  pages={3462-7}
}
Subtilisin Carlsberg was covalently modified with comb-shaped poly(ethylene glycol) (PM13). PM13-modified subtilisin (PM13-Sub) was readily solubilized in three different ionic liquids (ILs), i.e., [Emim][Tf2N], [C2OC1mim][Tf2N] and [C2OHmim][Tf2N]. Analysis of homogeneous enzymatic reactions in the ILs revealed that PM13-Sub exhibited excellent catalytic performance while the native enzyme suspended in ILs showed no activity. Hydrophobicity of ILs slightly affected enzyme activity, and the… CONTINUE READING