Homogeneous assays for adenosine 5'-monophosphate-activated protein kinase.

@article{Li2003HomogeneousAF,
  title={Homogeneous assays for adenosine 5'-monophosphate-activated protein kinase.},
  author={Ying Li and Richard T. Cummings and Barry R Cunningham and Yuli Chen and Gaochao Zhou},
  journal={Analytical biochemistry},
  year={2003},
  volume={321 2},
  pages={151-6}
}
Allosteric activation of 5(')-AMP-activated protein kinase (AMPK) is currently of interest as an approach for the treatment of metabolic disorders because AMPK plays multiple roles in glucose and lipid metabolism. The availability of ultrafast, ultrasensitive, and robust assays suited to high-throughput screening (HTS) is key to obtaining small-molecule AMPK activators. In the absence of high-affinity and selective antiphospho Ser/Thr antibodies for AMPK substrates, we have developed two… CONTINUE READING