Homocysteine editing and growth inhibition in Escherichia coli.

  title={Homocysteine editing and growth inhibition in Escherichia coli.},
  author={Marta Sikora and Hieronim Jakubowski},
  volume={155 Pt 6},
In Escherichia coli homocysteine (Hcy) is metabolically converted to the thioester Hcy-thiolactone in ATP-consuming reactions catalysed by methionyl-, isoleucyl- and leucyl-tRNA synthetases. Here we show that growth inhibition caused by supplementation of E. coli cultures with Hcy is accompanied by greatly increased accumulation of Hcy-thiolactone. Energy dissipation for Hcy editing increases 100-fold in the presence of exogenous Hcy and reaches one mole of ATP unproductively dissipated for Hcy… CONTINUE READING