Homocitrate synthase from yeast.

@article{Tucci1972HomocitrateSF,
  title={Homocitrate synthase from yeast.},
  author={A. Tucci and L. Ceci},
  journal={Archives of biochemistry and biophysics},
  year={1972},
  volume={153 2},
  pages={
          742-50
        }
}
  • A. Tucci, L. Ceci
  • Published 1972
  • Biology, Medicine
  • Archives of biochemistry and biophysics
Homocitrate synthase activity has been partially purified from yeast. Homocitrate synthase activity was sensitive to inhibition by lysine both in vivo and in vitro only at high concentrations of lysine. Two enzyme activities have been demonstrated in several strains of yeast by isoelectric focusing. The two enzymes were equally sensitive to inhibition by lysine. One enzyme activity was repressed to a greater extent when the culture medium was supplemented with excess lysine. 
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Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae
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In Saccharomyces cerevisiae, most of the LYS structural genes have been identified except the genes encoding homocitrate synthase and α‐aminoadipate aminotransferase, which could be a major element in the control of αAASA supply. Expand
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Insensitivity of Homocitrate Synthase in Extracts of Penicillium chyrosogenum to Feedback Inhibition by Lysine
We previously reported that lysine inhibits in vivo homocitrate synthesis in the lysine bradytroph, Penicillium chrysogenum L1, and that such feedback inhibition could explain the known lysineExpand
Some Properties of Lysine-biosynthesizing Enzymes of Candida utilis
TLDR
It is shown that the synthesis of the lysine biosynthetic enzymes is not regulated, and the regulatory properties of these enzymes were investigated and compared with the regulatory patterns found in other yeast species. Expand
Inhibition and repression of homocitrate synthase by lysine in Penicillium chrysogenum
TLDR
In vitro studies indicated that L-lysine feedback inhibits and represses homocitrate synthase both in low- and high-penicillin-producing strains, including Penicillium chrysogenum. Expand
Regulation of the lysine biosynthesis in Pichia guilliermondii
TLDR
The regulatory properties of four enzymes involved in the lysine biosynthesis of Pichia guilliermondii were investigated and it is shown that only the synthesis of homocitrate synthase is weakly repressed by L-lysine. Expand
A kinetic study of homocitrate synthetase activity in the yeast Saccharomycopsis lipolytica.
TLDR
The results do not support the existence of isoenzymes at the level of homocitrate synthetase in this yeast and two types of mutations which modify these inhibition patterns without affecting the catalytic activity are described. Expand
In Saccharomyces cerevisae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p.
TLDR
It is demonstrated that Saccharomyces cerevisiae possesses two genes, LYS20 and LYS21, encoding two homocitrate synthase isoenzymes which are located in the nucleus, and the data are consistent with the conclusion that inhibition by lysine of Lys14p activation results from the control of alphaAASA production through the feedback inhibition of homocite synthase activity. Expand
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The inhibitory effect of lysine on the formation of HC in both whole cells and cell-free extracts is indicative of the functional existence of a feedback control mechanism in the pathway for lysinesine biosynthesis in yeast. Expand
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TLDR
The excretion of homocitric acid by a lysine-requiring yeast mutant and its conversion into lysin convincingly establish the biosynthesis of lysines in yeast via the previously proposed scheme involving homologues of the citric acid cycle. Expand
Control of lysine biosynthesis in yeast.
  • A. Tucci, L. Ceci
  • Biology, Medicine
  • Archives of biochemistry and biophysics
  • 1972
TLDR
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TLDR
Accumulation of a-ketoglutaric acid in two nonallelic yeast mutants (Ly7 and Ly4) requiring lysine has been demonstrated, and data suggest that there are two different enzymic steps to convert homocitrate to homisocitrates. Expand
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TLDR
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Abstract Accumulation of tricarboxylic acids related to lysine biosynthesis has been demonstrated in a yeast mutant, Ly12, which requires lysine as a growth factor in synthetic medium but canExpand
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TLDR
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Feedback Inhibition of Lysine Biosynthesis in Yeast
  • A. Tucci
  • Medicine, Biology
  • Journal of bacteriology
  • 1969
The pathway for the biosynthesis of lysine is feedback-inhibited by lysine in growing yeast.
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TLDR
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein. Expand
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