• Corpus ID: 220646503

Holographic immunoassays.

@article{Snyder2020HolographicI,
  title={Holographic immunoassays.},
  author={Kaitlynn Snyder and Rushna Quddus and Andrew D Hollingsworth and Kenneth S. Kirshenbaum and David G. Grier},
  journal={arXiv: Soft Condensed Matter},
  year={2020}
}
The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by measuring the kinetics of irreversible binding for the antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) as they attach to micrometer-diameter colloidal beads coated with protein A. These measurements yield the antibodies' binding rates and can be… 

Figures from this paper

References

SHOWING 1-9 OF 9 REFERENCES

Generalized Lorenz-Mie Theories

Background in Maxwell's Electromagnetism and Maxwell's Equations.- Resolution of Special Maxwell's Equations.- Generalized Lorenz-Mie Theories in the Strict Sense, and other GLMTs.- Gaussian Beams,

Scattering

This course addresses the challenges that arise with light scattering in computer graphics and computer vision, including appearance modeling, underwater imagery, vision in bad weather, and measurement techniques.

Biomed

  • Opt. Express , submitted for publication
  • 2020

Front

  • Philip D. Plowright
  • 2020 Fourth World Conference on Smart Trends in Systems, Security and Sustainability (WorldS4)
  • 2020

Absorption and scattering of light by small particles

Soft Matter 16

  • 891 7
  • 2020

Hilaire

  • J. Mol. Recognit 24, 945
  • 2011

Cold Spring Harbor Protocols 2019

  • pdb
  • 2019

Immunol

  • 109, 581
  • 2002