• Corpus ID: 220646503

Holographic immunoassays.

@article{Snyder2020HolographicI,
  title={Holographic immunoassays.},
  author={Kaitlynn Snyder and Rushna Quddus and Andrew D Hollingsworth and Kenneth S. Kirshenbaum and David G. Grier},
  journal={arXiv: Soft Condensed Matter},
  year={2020}
}

• Kaitlynn Snyder, Rushna Quddus, +2 authors D. Grier
• Published 18 July 2020
• Biology
• arXiv: Soft Condensed Matter

The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by measuring the kinetics of irreversible binding for the antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) as they attach to micrometer-diameter colloidal beads coated with protein A. These measurements yield the antibodies' binding rates and can be inverted to obtain the concentration of antibodies in solution. Holographic molecular binding assays therefore can be used to perform fast quantitative immunoassays that are complementary to conventional serological tests.