Hog cholera IV. Detection of the virus in tissue culture preparations by the fluorescent antibody technique.

@article{Robertson1965HogCI,
  title={Hog cholera IV. Detection of the virus in tissue culture preparations by the fluorescent antibody technique.},
  author={A. V. Robertson and A. S. Greig and M L Appel and Alexis Girard and G L Bannister and Pierre A. Boulanger},
  journal={Canadian journal of comparative medicine and veterinary science},
  year={1965},
  volume={29 9},
  pages={234-41}
}
The fluorescent-antibody technique was employed for detection of hog cholera virus in tissue cultures inoculated with spleens of infected animals. As controls, cultures were also inoculated with material from normal swine and from those infected with other agents. In the first series 71 of 73 infected spleens, or 97 per cent, were detected. There were no false positive reactions among the controls. Results obtained with the second series of pigs showed that spleens collected during advanced… CONTINUE READING