Hoechst 33258 as a vital stain for plant cell protoplasts

@article{Meadows1981Hoechst3A,
  title={Hoechst 33258 as a vital stain for plant cell protoplasts},
  author={M. G. Meadows and Ingo Potrykus},
  journal={Plant Cell Reports},
  year={1981},
  volume={1},
  pages={77-79}
}
A method for staining nuclei in unfixed protoplasts using Hoechst 33258 is described. It was found that the dye was not taken up by all protoplasts except in the presence of a detergent and that the staining of the nuclei was pH- and concentration-dependent. The use of vital fluorescent probes with plant protoplasts may be helpful for the identification, selection and subsequent culture of hybrid fusion products in automatic cell sorting systems. 

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