Hla-do-specific Alleles Associated with Celiac Disease (cd) Are Expressed in the Small Intestinal Mucosa (sm)

Abstract

Previous studies at DNA and product level of B cell lines on CD patients have shown a strong association between CD and the HLA-DQα2.3 and HLA-DQ β2.7 alleles1. The rat monoclonal SFR-20 DQα5 which specifically recognizes the HLA-DQα2.3 specificity2 and the monoclonal directed against the HLA-DQβ2.7 specificity (MC Mazzili, Italy) have been used to detect the expression of these specificities at the SIM in 7 CD patients and 6 non-CD patients. The mouse monoclonal SPV-L3 against HLA-DQ backbone was used as positive immunoperoxidase control. An immunoperoxidase technique on frozen tissue sections of jejunal biopsy specimen was used.Results: Positive specimens showed an infiltration of positive lymfocytes and histiocytes in the lamina propria. Positive results at the intestinal level correlated with HLA typing of the patients and controls. The epithelial cells were negative.Conclusion: The results show that at intestinal level the HLA-DQ specific alleles associated with CD are not expressed in the epithelial cells but abundantly present in lamina propria cells of celiac patients. This distribution may support the hypothesis that these DQ molecules are involved in the regulation of the intestinal immune response to gluten.1. Roep etal. Hum Immunol 1988; 23:271–92. Amar etal. J Immunol 1987; 138:3986–90

DOI: 10.1203/00006450-198909000-00058

Cite this paper

@article{Schweizer1989HladospecificAA, title={Hla-do-specific Alleles Associated with Celiac Disease (cd) Are Expressed in the Small Intestinal Mucosa (sm)}, author={Joachim J. Schweizer and H L Mearin and A. S. Pe{\~n}a and George Johan Arnold Offerhaus and Enno J Dreef and Bart O. Roep and R. E. Bontrop and C H B W Lamers and Leonard J. Dooren and Philip J. Hoedemaeker}, journal={Pediatric Research}, year={1989}, volume={26}, pages={273-273} }