Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes

@inproceedings{Yin2014HittingTT,
  title={Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes},
  author={Xingyu Yin and Alexander Scalia and Ludmila Leroy and Christina M. Cuttitta and Gina M. Polizzo and Daniel L. Ericson and Christian Roessler and Olven Campos and Millie Y. Ma and Rakhi Agarwal and Rick Jackimowicz and Marc Allaire and Allen M. Orville and Robert M. Sweet and Alexei Soares},
  booktitle={Acta crystallographica. Section D, Biological crystallography},
  year={2014}
}
Acoustic droplet ejection (ADE) is a powerful technology that supports crystallographic applications such as growing, improving and manipulating protein crystals. A fragment-screening strategy is described that uses ADE to co-crystallize proteins with fragment libraries directly on MiTeGen MicroMeshes. Co-crystallization trials can be prepared rapidly and economically. The high speed of specimen preparation and the low consumption of fragment and protein allow the use of individual rather than… CONTINUE READING

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