Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA.

@article{Lin1976HistonesBM,
  title={Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA.},
  author={Stephen YuHsiang Lin and Dr. Henry C. Lin and Arthur D. Riggs},
  journal={Nucleic acids research},
  year={1976},
  volume={3 9},
  pages={
          2183-91
        }
}
Using a membrane filter assay, we have obtained results from both kinetic and competition experiments indicating that histones bind more strongly to bromodeoxyuridine-substituted DNA than to normal DNA. At 37 degrees C in our standard buffer of 0.2 M ionic strength, the rate of dissociation of histones H1, H2, and h4 from BrdU-substituted DNA is respectively 7, 4, and 2 times slower than it is from normal DNA. Competition experiments show an even greater difference between BrdU-substituted and… CONTINUE READING

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