Histone variant H2A.Z and RNA polymerase II transcription elongation.

  title={Histone variant H2A.Z and RNA polymerase II transcription elongation.},
  author={Maria Soledad Santisteban and Mingda Hang and M Mitchell Smith},
  journal={Molecular and cellular biology},
  volume={31 9},
Nucleosomes containing histone variant H2A.Z (Htz1) serve to poise quiescent genes for activation and transcriptional initiation. However, little is known about their role in transcription elongation. Here we show that dominant mutations in the elongation genes SPT5 and SPT16 suppress the hypersensitivity of htz1Δ strains to drugs that inhibit elongation, indicating that Htz1 functions at the level of transcription elongation. Direct kinetic measurements of RNA polymerase II (Pol II) movement… CONTINUE READING