Histone-like protein: a novel method for measuring stress in fish.

@article{Robinette2001HistonelikePA,
  title={Histone-like protein: a novel method for measuring stress in fish.},
  author={David W. Robinette and Edward J. Noga},
  journal={Diseases of aquatic organisms},
  year={2001},
  volume={44 2},
  pages={97-107}
}
We assessed the effect of chronic stress using a group of potent, broad-spectrum antimicrobial polypeptides, called histone-like proteins (HLPs), which appear to be an important component of non-specific immunity in channel catfish Ictalurus punctatus skin. An enzyme-linked immunosorbent assay (ELISA) was developed to measure the predominant HLP (HLP-1) in channel catfish skin. Catfish were then exposed to a chronic stress consisting of overcrowding and elevated ammonia. Healthy unstressed fish… CONTINUE READING

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