Histone H1 preferentially binds to superhelical DNA molecules of higher compaction.

@article{Ivanchenko1997HistoneHP,
  title={Histone H1 preferentially binds to superhelical DNA molecules of higher compaction.},
  author={Maria G. Ivanchenko and Jordanka S. Zlatanova and Kensal E van Holde},
  journal={Biophysical journal},
  year={1997},
  volume={72 3},
  pages={1388-95}
}
In chromatin, the physiological amount of H1 is one molecule per nucleosome or, roughly, one molecule per 200 bp of DNA. We observed that at such a stoichiometry, H1 selectively binds to supercoiled DNA with magnitude of sigma > or = 0.012 (both negative and positive), leaving relaxed, linear, or nicked DNA molecules unbound. When negative and positive DNA… CONTINUE READING