Ultrastructural features of canine kidneys after preservation with hyperthermic solutions and subsequent reperfusion
Four different preserving fluids were studied for protective effect in a total of 33 isolated canine kidneys. The preservation method consisted in initial perfusion followed by 24 hours of hypothermic storage. Two electrolyte and two non-electrolyte solutions were tested. One of the electrolyte solutions was of the extracellular, the other of the intracellular type. The two non-electrolyte solutions were 5% human albumin and 10% Rheomacrodex. On the evidence of serial microscopic studies the Collins-4 solution of intracellular type allows the best viability: even the 24-hour specimens exhibited a normal renal structure. Of the non-electrolyte solutions Rheomacrodex revealed an adequate protective effect despite the production of a marked interstitial oedema and signs of dehydration involving the tubular cells. The 5% human albumin solution failed to preserve the integrity of renal structure for 24 hours. The electrolyte solution of extracellular type proved still less satisfactory, the glomerular loops and the tubular epithelium reveling signs of autolysis by the end of the 24-hour period.