Histological evidence: housekeeping genes beta-actin and GAPDH are of limited value for normalization of gene expression

@article{Lin2012HistologicalEH,
  title={Histological evidence: housekeeping genes beta-actin and GAPDH are of limited value for normalization of gene expression},
  author={Juntang Lin and Christoph Redies},
  journal={Development Genes and Evolution},
  year={2012},
  volume={222},
  pages={369-376}
}
Housekeeping genes are widely used as internal controls for gene expression normalization for western blotting, northern blotting, RT-PCR, etc. They are generally thought to be expressed in all cells of the organism at similar levels because it is assumed that these genes are required for the maintenance of basic cellular function as constitutive genes. However, real-time RT-PCR experiments revealed that their expression may vary depending on the developmental stage, type of tissue examined… CONTINUE READING