• Corpus ID: 56226963

Histological, ultrastructural and immunohistochemical comparison of immature rat testis cryopreserved with glycerol, propanediol and dimethylsulphoxide

  title={Histological, ultrastructural and immunohistochemical comparison of immature rat testis cryopreserved with glycerol, propanediol and dimethylsulphoxide},
  author={Ibtisam Khalaf Abd Ali and Khalid Kamil Kadhim and Anam Rashid Al-salihi},
  journal={Journal of entomology and zoology studies},
The morphological changes caused by cryopreservation of the testicular tissues with different cryoprotectants have been assessed here. Testes of thirty immature male rats divided into equal five groups; Group (A) as control fresh sample, group (B) with freezing media only. Different freezing protocol were used for other groups; group (C) dimethylsulphoxide (DMSO); group (D) Glycerol and group (E) propanediol (PROH). Light and transmission electron microscope in addition to the… 



Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants.

DMSO as a cryoprotectant (at a 0.7 mol/l concentration) proved to maintain the structure of testicular tissue, especially spermatogonia, after cryopreservation better than PrOH or glycerol.

Effects of various cryopreservation media and freezing–thawing on the morphology of rat testicular biopsies

It is concluded that freezing–thawing of testicular biopsies, and the cryopreservation medium, have a significant impact on the structure of the seminiferous epithelium, particularly on its basal compartment.

Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies.

It is concluded that the freezing and thawing of testicular biopsies causes similar morphological damage to testicular spermatozoa and frozen-thawed ejaculated spermatozosa.

A Histological and Morphometric Study of Monosodium Glutamate Toxic Effect on Testicular Structure and Potentiality of Recovery in Adult Albino Rats

Rats treated with MSG showed morphological and morphometric changes as decrease in testicular weight, decrease in tubular diameter, reduction in germinal epithelium height, increase in the spermatic count and abnormalities of sperms morphology, but normal structure of the testis was not regained even after six weeks of cessation of treatment.

Cryopreservation of cat testicular tissues: effects of storage temperature, freezing protocols and cryoprotective agents.

Cryopreservation of testicular tissue has become a part of gamete preservation in wild animal post-mortem. Using domestic cats as a model for wild felids, this study aimed to (i) investigate the

Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment.

Pre-pubertal testicular testicular tissue from boys facing gonadotoxic treatment survives cryopreservation, can be cryobanked and hopefully used for fertility preservation.

Characterization and Functionality of Proliferative Human Sertoli Cells

The results show that functional human Sertoli cells can be propagated in vitro from testicular cells isolated from adult testis and should have important applications in studying infertility, reproductive toxicology, testicular cancer, and spermatogenesis.

Freezing of testicular tissue as a minced suspension preserves sperm quality better than whole-biopsy freezing when glycerol is used as cryoprotectant.

Frozen-thawed testicular spermatozoa have been used successfully for ICSI, especially in cases of obstructive azoospermia with normal spermatogenesis. Fewer attempts, however, have been made to check