Histochemical staining following LacZ gene transfer underestimates transfection efficiency.

@article{Couffinhal1997HistochemicalSF,
  title={Histochemical staining following LacZ gene transfer underestimates transfection efficiency.},
  author={Thierry Couffinhal and Marianne Kearney and Alison Sullivan and Marcy Silver and Yukio Tsurumi and Jeffrey M. Isner},
  journal={Human gene therapy},
  year={1997},
  volume={8 8},
  pages={929-34}
}
Analysis of LacZ gene expression is conventionally inferred from blue staining that results from exposure of the transfected cells or tissue to the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). Such histochemical staining reports not whether the gene product is present or absent, but where it is active. We investigated the… CONTINUE READING