Histochemical demonstration of carbonic anhydrase activity

  title={Histochemical demonstration of carbonic anhydrase activity},
  author={Holger P. J. Hansson},
Freeze-dried frozen sections are floated on the surface of the freshly prepared incubation mixture (CoSO4 1.75 × 10−3 M, H2SO4 5.3 × 10−2 M, NaHCO3 1.57 × 10−2 M and KH2PO4 1.17 to 11.7 × 10−3 M; demonstration of weak activity requires high phosphate). A compound containing cobalt and phosphorous precipitates at carbonic anhydrase sites and is converted to CoS. Adequate staining requires only 2–10 minutes of incubation. Actazolamide inhibits the staining reaction in specific concentrations… CONTINUE READING
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