Histochemical demonstration of carbonic anhydrase activity

@article{Hansson1967HistochemicalDO,
  title={Histochemical demonstration of carbonic anhydrase activity},
  author={Holger P. J. Hansson},
  journal={Histochemie},
  year={1967},
  volume={11},
  pages={112-128}
}
Freeze-dried frozen sections are floated on the surface of the freshly prepared incubation mixture (CoSO4 1.75 × 10−3 M, H2SO4 5.3 × 10−2 M, NaHCO3 1.57 × 10−2 M and KH2PO4 1.17 to 11.7 × 10−3 M; demonstration of weak activity requires high phosphate). A compound containing cobalt and phosphorous precipitates at carbonic anhydrase sites and is converted to CoS. Adequate staining requires only 2–10 minutes of incubation. Actazolamide inhibits the staining reaction in specific concentrations… CONTINUE READING
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l~Ao: Immunological and kinetic properties of carbonic anhydrases from various tissues. Biochem, biophys

  • S N.
  • Acta (Amst.) (in press)
  • 1967
Highly Influential
6 Excerpts

liver and kidney tissues

  • H. W. DAVENPORT
  • Arch. Biochem
  • 1939
Highly Influential
5 Excerpts

On the non-specificity of histoehemical methods for carbonic anhydrase

  • T. F. MUTHER
  • Fed. Proc. 25,
  • 1966
1 Excerpt

KOR~O~E~: Histochemical demonstration of carbonic anhydrase activity in the eyes of rat and mouse

  • E. KORHOE, K L.
  • Acta ophthah (Kbh.)
  • 1965

HYYPPX: A histochemical study of carbonic anhydrase in some parts of the mouse brain

  • L. K. KORIONEN, E. NXXTXEN
  • Acta histochem. (Jena)
  • 1964

Comparison by direct measurement of sulfonamide carbonic anhydrase inhibition in whole cells and in enzyme solutions. In: Drugs and enzymes

  • WISTI, J P.
  • Proc. of the 2nd Internat. Pharmacological…
  • 1963

ROUGHTON: The activity of concentrated solutions of carbonic anhydrase

  • W. W. FORREST, W F.J.
  • Biochim. biophys. Acta (Amst.)
  • 1963

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